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为了解青岛地区丙型肝炎病毒(HCV)基因型分布情况以及HCV5’NC和HCV-NS5b两区域分型结果间的差异。本文对采自147例各型内肝患者和7例有偿供血者的共154份HCV RNA阳性血清,同时进行了HCV5’NC和HCV-NS5b两个区域酶切分型研究结果显示,两区域的HCVⅡ(1B)型感染分别占82.5％和83.1％;在各临床类型中,肝硬变患者的HCVⅡ(1b)型占91.3％,高于其它临床类型.提示HCVⅡ(1b)型为青岛地区HCV感染的优势株,并且可能和肝病的进展密切相关.研究结果同时还表明,HCV-5’NC区和HCV-NS5b区酶切分型结果间具有良好的相互对应关系。
To understand the distribution of hepatitis C virus (HCV) genotypes in Qingdao and the differences between the genotyping results of HCV5’NC and HCV-NS5b regions. A total of 154 HCV RNA positive sera collected from 147 patients with liver disease in each type and 7 compensated blood donors were collected and the two regions of HCV5’NC and HCV-NS5b were digested and typed. The results showed that the two regions The HCV Ⅱ (1B) infection accounted for 82.5% and 83.1% respectively, and in all clinical types HCV Ⅱ (1b) accounted for 91.3% of patients with cirrhosis, which was higher than other clinical types. And may be closely related to the progress of liver disease.The results of the study also showed that there was a good reciprocal relationship between the results of restriction enzyme digestion of HCV-5’NC region and HCV-NS5b region.