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目的:通过分析清风藤属9种药用植物的叶表皮显微特征,探讨此方案鉴别不同清风藤药用植物的意义。方法:采集清风藤属的凹萼清风藤、平伐清风藤、尖叶清风藤、四川清风藤、灰背清风藤、鄂西清风藤、两色清风藤、多花清风藤、小花清风藤等九种不同用药植物样本,肉眼观察其外观形态特征,记录其在显微镜下的上下叶表皮特征及表皮细胞周长均值差异。结果:1除平伐清风藤及灰背清风藤上表皮垂周壁呈稍弯曲形态且上表皮细胞呈不规则形外,其他7种清风藤属植物上表皮垂周壁均呈平直形态、上表皮细胞也呈规则的多角形;2凹萼清风藤、尖叶清风藤、平伐清风藤、灰背清风藤下表皮垂周壁呈深波状弯曲形态,两色清风藤呈略弯曲或平直形态,其余植物均呈浅波状弯曲形态;3仅尖叶清风藤上、下表皮有单细胞非腺毛,多花清风藤仅上表皮有,四川清风藤则仅下表皮有,其余植物叶表面均无单细胞非腺毛;49种清风藤属药用植物周长均值差异较大,四川清风藤上表皮细胞周长最长,但下表皮细胞周长最短;小花清风藤上表皮细胞周长最短;凹萼清风藤下表皮细胞周长则最长。结论:将清风藤属的9种药物植物放置于显微镜下,能根据其叶表皮细胞特征及周长平均值特点等有效判断、鉴别其种类,是清风藤属植物及药材分类鉴定的新思路,值得推广使用。
OBJECTIVE: To analyze the microscopic characteristics of the leaf epidermis of nine medicinal plants of the genus Purpurea, and to explore the significance of this protocol in identifying different medicinal plants of the genus Purpurea. Methods: The bark of Qingchen rattan was collected from Qingchen rattan, Pinghua Qingfeng rattan, Jianpi Qingchen rattan, Sichuan Qingfeng rattan, Qingpi rattan, Qingchen rattan, Qingchen rattan, Qingchen rattan, Qingchen rattan and other flowers The morphological characteristics of nine different medicinal plants were observed visually, and the differences of the upper and lower epidermis characteristics and the average epidermal cell perimeter under the microscope were recorded. Results: 1 The sagittal wall of the upper epidermis was flat and the upper epidermis was flat except the upper wall of the upper epidermis was slightly curved and the upper epidermis was irregular. Cells are also regular polygons; 2 calyx broom cane, sharp leaf breeze rattan, Ping rattan broom, raccoon bark lower epidermal perineural wall was deep wave-like curved shape, two-color breeze rattan slightly curved or straight shape, the rest Plants showed a shallow wavy curved shape; only the tip of the leaves breeze rattan, the lower epidermis with single-cell non-glandular hair, spend more than the upper bark Qingchen only, Sichuan breeze only the lower dermis, there is no single leaf surface Cell non-glandular hair; 49 kinds of medicinal plants broomcrest medicinal plants differences in the average circumference of the larger, Sichuan broomcrest upper epidermal cell perimeter longest, but the lower epidermal cell circumference the shortest; The calyx cingulate ramulus epidermal cells perimeter is the longest. CONCLUSION: Nine species of Radix genera are placed under the microscope and can be effectively judged according to the characteristics of their epidermal cells and the average of their periforms to identify their species. It is a new idea for the classification and identification of the species of Radix Genus and herbs, Worth to promote use.