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根据绿色荧光蛋白的发光原理,采用聚乙二醇与聚甲基丙烯酸甲酯的两亲性两嵌段聚合物通过自组装包覆生色团的方式,模拟了绿色荧光蛋白发光,考察了组装行为对光学性能的影响,并将其用于细胞成像.通过核磁共振、高分辨质谱、傅里叶变换红外光谱、凝胶渗透色谱、紫外-可见吸收光谱及荧光光谱等表征了生色团分子和聚合物的结构及性能.生色团紫外最大吸收在371 nm,荧光最大发射峰在428 nm.聚合物和生色团进行组装后,其紫外吸收消失,而最大荧光发射峰强度大大增强,且发生了约70 nm的红移,这是因为组装使得生色团的自由旋转受到了限制,且生色团共平面性增加.动态光散射(DLS)和透射电镜(TEM)证明了纳米粒子的结构和尺寸.由于尺寸适合且具有较好的荧光性能,纳米粒子成功应用于细胞成像.这种绿色荧光蛋白生色团的简单自组装方式在生物成像领域具有良好应用前景.
According to the principle of luminescence of green fluorescent protein, the amphiphilic diblock polymer of polyethylene glycol and polymethylmethacrylate was used to simulate the luminescence of green fluorescent protein through the self-assembly of the chromophore. Behavior on the optical properties and its application to cell imaging.Highly characterized by chromotomes such as nuclear magnetic resonance, high resolution mass spectrometry, Fourier transform infrared spectroscopy, gel permeation chromatography, UV-Vis absorption and fluorescence spectroscopy And the structure and properties of the polymer.The maximum UV absorption of the chromophore is at 371 nm and the maximum fluorescence emission peak is at 428 nm.The ultraviolet absorption disappears and the maximum fluorescence emission intensity increases greatly after the polymer and the chromophore are assembled, And a redshift of about 70 nm occurred because the assembly allowed the free rotation of the chromophore to be limited and the coplanarity of the chromophore increased.Dynamic light scattering (DLS) and transmission electron microscopy (TEM) demonstrated that the nanoparticle Due to its size and good fluorescence properties, nanoparticles have been successfully used in cell imaging.The simple self-assembly of this green fluorescent protein chromophore has the potential in the field of bioimaging Good prospects.