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目的:探讨稀土元素镧、铈对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响。方法:冬凌草均匀愈伤组织在添加不同浓度La3+或Ce3+的培养基中培养,通过质量法测定愈伤组织鲜质量、绘制生长曲线,HPLC测定甲素、乙素含量。结果:CeCl3.7H2O浓度为1μmol.L-1时,愈伤生物量最高,是空白条件下的2.4倍。空白及对照条件下甲素含量均为0;空白条件下乙素含量最低为0.11%,随着Ce3+浓度升高而升高,当浓度为10μmol.L-1时高达0.58%;乙素随着La3+浓度升高而降低,当浓度为0.1μmol.L-1时含量高达0.44%。结论:0～10μmol.L-1的La3+及Ce3+均有利于冬凌草愈伤生物量的积累及乙素的合成,对甲素的合成无影响,将CeCl3.7H2O浓度10μmol.L-1作为最佳条件选择。
OBJECTIVE: To investigate the effects of lanthanum and cerium on the callus growth and the content of oridonin and acetonide in Rubescens. Methods: Callicarpa cocultipodes were cultured in medium supplemented with different concentrations of La3 + or Ce3 +. The fresh weight of callus was determined by mass spectrometry. The growth curve was drawn. The contents of A and B were determined by HPLC. Results: When the concentration of CeCl3.7H2O was 1 μmol·L-1, the callus biomass was the highest, which was 2.4 times of the blank. The contents of alanine in blank and control were all 0; the lowest content of bilirubin in blank was 0.11%, increased with the increase of Ce3 + concentration, up to 0.58% at a concentration of 10μmol.L-1; The concentration of La3 + increased and decreased, when the concentration was 0.1μmol.L-1, the content was as high as 0.44%. CONCLUSION: Both La3 + and Ce3 + of 0 ~ 10μmol.L-1 are beneficial to the accumulation of callus biomass and synthesis of B in A. rubescens and have no effect on the synthesis of A. The CeCl3.7H2O concentration of 10μmol.L-1 Best choice of conditions.