EXPRESSION IN Yersinia enterocolitica OF K99 ANTIGEN GENE OF Escherichia coli

• 作 者: 孙午良 范云六
• 机 构: Laboratory of Molecular Biology, Biotechnology Research Center, Chinese Academy of Agricultural Scie
• 出 处: Science in China,Ser.B
• 发表日期: 1988年10期
• 关键词: polyacrylamide plasmid Proteins serotype witht heating Plasmid cloning determina

The expression of the determinant for the K99 antigen of Escherichia coli in Yersinia enterocolitica avirulent transconjugants D29(pFS239) and L15(pFS239) was confirmed by means of SDS-polyacrylamide gel electrophoresis, double immunodiffusion and Western blotting. The conditions for the expression of K99 antigen genes in Y. enterocolitica and in E. coli were compared. The K99 antigen protein expressed in Y. enterocolitica in the form of pili on the surface of the cells was discussed. This article offers the first description on the expression in Y. enterocolitica of gene from organism other than Yersinia spp.

The expression of the determinant for the K99 antigen of Escherichia coli in Yersinia enterocolitica avirulent transconjugants D29 (pFS239) and L15 (pFS239) was confirmed by SDS-polyacrylamide gel electrophoresis, double immunodiffusion and Western blotting. The conditions for the expression of K99 antigen genes in Y. enterocolitica and in E. coli were compared. The K99 antigen protein expressed in Y. enterocolitica in the form of pili on the surface of the cells was discussed. This article provides the first description on the expression in Y. enterocolitica of gene from organism other than Yersinia spp.