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目的：探讨巨噬细胞集落刺激因子（M－CSF）、白细胞介素-1（IL－1）和肿瘤坏死因子α（TNF－α）在骨巨细胞瘤（GCT）形成及其溶骨中的作用。方法：运用免疫组化方法和细胞培养技术，观察M－CSF、IL－1和TNF-α在18例GCT中的表达及其对GCT溶骨的影响。结果：在GCT中，M－CSF主要由纤维母细胞样基质细胞（FC）分泌；巨噬细胞样基质细胞（HC）主要分泌IL－1和TNF－α，少数多核巨细胞（MGC）也可产生TFN－α。外源性的TNF－α。外源性的能加强GCT中FC对骨质的破坏。结论：GCT中HC和MGC分泌的IL－1和TNF－α，可以刺激FC的溶骨和浸润性的生长，从而增强GCT侵袭性的生物学行为。而FC产生的M－CSF可能促进厂GCF中MGC的形成。
Objective: To investigate the role of macrophage colony-stimulating factor (M-CSF), interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) in the formation of bone giant cell tumor (GCT) and its osteolysis effect. METHODS: Immunohistochemistry and cell culture techniques were used to observe the expression of M-CSF, IL-1 and TNF-α in 18 cases of GCT and its effect on the osteolysis of GCT. RESULTS: In GCT, M-CSF was mainly secreted by fibroblast-like stromal cells (FC); macrophage-like stromal cells (HC) mainly secreted IL-1 and TNF-alpha, and a few multinucleated giant cells (MGC) were also available. TFN-α is produced. Exogenous TNF-α. Exogenous can enhance the destruction of bone by FC in GCT. Conclusion: IL-1 and TNF-α secreted by HC and MGC in GCT can stimulate the osteolytic and invasive growth of FC, thus enhancing the biological behavior of GCT invasiveness. The M-CSF produced by FC may promote the formation of MGC in the plant GCF.