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目的 探讨干扰素的体外抗乙型肝炎病毒作用。方法 以HepG2 2 ,2 ,15为细胞模型 ,实验共观察 9d ,于第 3、6、9d收集培养上清行HBsAg定量检测 ;收集细胞提取总RNA ,行RT -PCR及荧光实时定量PCR测细胞内乙肝病毒mRNA水平。结果 实验第 3d及第 6d ,对照组和各实验组之间HBsAg及病毒mRNA水平无显著性差异 ;实验第 9d ,各实验组HBsAg及病毒mRNA水平均较对照组明显降低 ,α干扰素浓度为 5 0 0IU/ml抑制作用达最大。结论 干扰素在实验浓度下可抑制HepG2 2 ,2 ,15细胞内乙肝病毒基因的转录和表达 ,具有直接抑制乙肝病毒复制作用
Objective To investigate the anti-hepatitis B virus effect of interferon in vitro. Methods HepG2 2, 2 and 15 were used as the cell model. The cells were observed for 9 days. HBsAg was harvested on the 3rd, 6th and 9th day. The total RNA was collected and the cells were harvested for RT-PCR and real-time quantitative PCR Internal Hepatitis B virus mRNA levels. Results On the 3rd and 6th day after the experiment, there was no significant difference in the levels of HBsAg and virus mRNA between the control group and the experimental groups. On the 9th day after the experiment, the HBsAg and the mRNA levels in the experimental groups were significantly lower than those in the control group 5 0 0IU / ml maximum inhibitory effect. Conclusions Interferon can inhibit the transcription and expression of Hepatitis B virus gene in HepG2 2, 2 and 15 cells under the experimental concentration, which has a direct inhibitory effect on the replication of hepatitis B virus