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目的:评价阿司匹林对糖尿病大鼠肾缺血再灌注损伤后Cystatin C(蛋白酶抑制肽C)的影响。方法:32只成年Sprague-Dawley大鼠经链脲霉素(streptozotocin,STZ)腹腔注射建立糖尿病模型后随机分为4组,实验组分别经胃灌注10 mg/kg、20 mg/kg、30 mg/kg的阿司匹林,对照组灌注等量生理盐水15 d后建立肾缺血30 min再灌注2 h模型。抽取动脉血用ELISA法检测Cystatin C水平,取肾脏做病理切片和免疫组化检测。结果:各实验组血清Cystatin C水平明显低于对照组(P<0.05),实验组之间差异不显著(P>0.05)。HE染色实验组与对照组未见明显组织病理学差异。免疫组化显示对照组Cystatin C蛋白表达增多,而实验组表达不显著。结论:低剂量阿司匹林降低STZ诱导的糖尿病大鼠肾缺血再灌注后血浆Cystatin C水平,具有肾保护作用。
PURPOSE: To evaluate the effect of aspirin on Cystatin C (protease inhibitor C) after renal ischemia-reperfusion injury in diabetic rats. Methods: Thirty-two adult Sprague-Dawley rats were randomly divided into four groups after intraperitoneal injection of streptozotocin (STZ). The experimental groups were given gastric perfusion with 10 mg / kg, 20 mg / kg, 30 mg / kg of aspirin, the control group was injected with saline for 15 days, and the model of renal ischemia for 30 minutes and reperfusion for 2 hours was established. Arterial blood samples were collected for determination of Cystatin C by ELISA. Pathological sections and immunohistochemistry were performed on the kidneys. Results: Serum levels of Cystatin C in each experimental group were significantly lower than those in the control group (P <0.05). There was no significant difference between the experimental groups (P> 0.05). There was no obvious histopathological difference between HE staining group and control group. Immunohistochemistry showed that Cystatin C protein expression increased in the control group, but the experimental group was not significant. CONCLUSION: Low-dose aspirin can reduce the plasma Cystatin C level after renal ischemia / reperfusion in STZ-induced diabetic rats and has a protective effect on kidney.