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目的:寻找马蓝叶与其易淆品路边青叶的鉴别特征,建立有效的鉴别方法。方法:LMVP(叶形态-脉序图谱鉴别法),QAERM(定量分析评价鉴别中药品种真伪方法的可靠性方法)。结果:主要区别点:马蓝叶的叶基渐狭下延,干叶深暗蓝绿色、暗棕绿色,具梭状小点,无腺点或局部有小腺点,叶柄与中脉的界限不明显,最末二级脉的下方有三级脉从中脉发出;路边青的叶基不下延,干叶黄绿色、绿色、绿黄色、黄棕色,无梭状小点,密布腺点,叶柄与中脉的界限明显,最末二级脉的下方无三级脉从中脉发出。同时以上述6组区别点为指标,鉴别结果的正确性(AC)=98.5%～99.2%;重复性为符合率(ARO)=97.7%,一致性强度(Kappa)=0.95。结论:建立的“叶形态-脉序图谱鉴别法”可准确鉴别马蓝叶及易淆品路边青叶。操作简便、快速、价廉、可靠性强。
Objective: To find out the distinguishing characteristics of Malanye leaves and its obstructing products, and to establish an effective identification method. Methods: LMVP (leaf morphology - pulse sequence mapping method), QAERM (quantitative analysis to evaluate the authenticity of traditional Chinese medicine methods to determine the reliability of methods). Results: The main difference is that the leaf base of Malan leaves is narrowed down, the leaves are dark blue-turquoise, dark brown-green, with a fusiform dot, no gland or some small glandular points. The boundaries of petiole and midrib are not Obviously, there are three veins beneath the last two veins, which are emanating from the midrib; the green leafy leaves on the roadside are not extended, dry leaves yellowish green, green, yellowish green, yellowish brown, shuttleless dots, dense glandular points, With the obvious boundaries of the midrib, the bottom of the last two veins no three veins issued from the midrib. At the same time, the correctness of the identification results (AC) was 98.5% ~ 99.2%. The repeatabilities were ARO = 97.7% and Kappa = 0.95. Conclusion: The established “leaf morphological - pulse sequence identification method” can be accurately identified blue leaves and easy to confuse oblate leaves. Easy to operate, fast, inexpensive, reliable.