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为了鉴定东北地区异育银鲫Carassius auratus gibelio感染的病原体是否为洪湖碘泡虫Myxobolus honghuensis,在吉林省查干湖某异育银鲫养殖基地获取病鱼,采用病鱼体外观察、虫体形态学观察与可量形状测定,对该寄生虫进行了初步鉴定,并对该寄生虫的18S r DNA进行克隆、测序与生物信息学分析。结果表明:病鱼症状具有典型的碘泡虫感染特征;可量形状测定结果表明,虫体外观与尺寸均与洪湖碘泡虫接近;生物信息学分析表明,该虫的18S r DNA与Gen Bank中登录号为JF340216的洪湖碘泡虫同源性达到99.3%;系统发育树显示,与已知洪湖碘泡虫为同一进化分支,确定该病为洪湖碘泡虫感染。本研究中,首次报道了东北地区一例早期感染洪湖碘泡虫的异育银鲫病例,研究结果可为碘泡虫的防治提供参考。
In order to identify whether the causative agent of Carassius auratus gibelio infection in northeast China was Myxobolus honghuensis, an diseased fish was obtained from a breeding base of allogynogenetic silver crucian carp in Chagan Lake, Jilin Province. Observation and measurement of measurable shape, preliminary identification of the parasite, and the parasite 18S r DNA cloning, sequencing and bioinformatics analysis. The results showed that the symptoms of diseased fish had the typical characteristics of Iodine-Bubbling infection. The measurable shape of the diseased fish showed that the appearance and size of the diseased larvae were close to that of Ixodon in Honghu. Bioinformatics analysis showed that 18S rDNA The accession number JF340216 Honghu Ipomoea homophilum 99.3%; phylogenetic tree shows that known Honghu iodine worm for the same evolutionary branch, to determine the disease is Honghu iodophyllum infection. In this study, we reported for the first time a case of allogynogenetic crucian carp (Carassius auratus) infected early in Honghu Lake in Northeast China. The results provide a reference for the prevention and treatment of Iodomonad.