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采用镀铜镉还原法检测肺癌、良性肺病和肺部正常者血清及支气管冲洗液(BWF)中一氧化氮(NO)水平,并对 BWF 中 NO 与 T 淋巴细胞亚群的相关性进行了分析。结果显示,肺癌组 BWF 中 NO 水平明显高于良性肺病组及正常癌组(P<0.01),但肺癌组血清 NO 水平与良性肺病组比较无差别(P>0.1);肺癌组 BWF 与血清 NO 水平之间呈正相关(P<0.01):Ⅲ、Ⅳ期肺癌血清 NO 明显高于Ⅰ、Ⅱ期肺癌和良性肺病组(P<0.01);NO 水平与肺癌组织学类型无关。BWF 中 NO 水平与 CD_4/CD_8比值呈明显负相关(r=-0.67,P<0.01).以上结果提示在肺癌免疫研究中,NO 水平的增加可能主要起局部免疫抑制作用。
Determination of nitric oxide (NO) levels in serum and bronchial washing fluid (BWF) in patients with lung cancer, benign lung disease, and normal lungs using a cadmium plated copper reduction method and analysis of the correlation between NO and T-lymphocyte subsets in BWF . The results showed that the NO level in BWF in lung cancer group was significantly higher than that in benign lung disease group and normal cancer group (P<0.01), but there was no difference in serum NO level between lung cancer group and benign lung disease group (P>0.1); BWF and serum NO in lung cancer group There was a positive correlation between levels (P<0.01): NO levels in stage III and IV lung cancer were significantly higher than those in stage I and II lung cancer and benign lung disease groups (P<0.01); NO levels were not associated with the histological type of lung cancer. The level of NO in BWF was significantly negatively correlated with the ratio of CD_4/CD_8 (r=-0.67, P<0.01). The above results suggest that the increase of NO level in lung cancer immunity may mainly play a role in local immunosuppression.