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This study was conducted to explore the relationship between glutathione metabolism and tobacco mosaic virus( TMV) resistance,using a TMV resistant tobacco cultivar Yuyan 8 and another tobacco cultivar NC89 which has the same genetic background with Yuyan 8 as the experimental materials. Bioinformatics analysis on the transcriptome data obtained from high-throughput sequencing revealed that among pathways enriched with differentially expressed genes,glutathione metabolic pathway was found in both cultivars infected with TMV,and glutathione metabolism was enhanced in Yuyan 8. We detected the changes in the transcription of glutathione S-transferase( GST) which is a key enzyme in glutathione metabolic pathway with quantitative PCR,the enzyme activity of GST and the content of glutathione( GSH) with spectrophotometry,and the expression levels of the genes that are related to TMV resistance or involved in photosynthesis. The results showed that GST gene expression,enzyme activity and GSH content were all increased by TMV infection in both cultivars,and the increments in Yuyan 8 were more significant. The expression of GST and other genes relate to TMV resistance were verified under different sulfur conditions. The results showed that the expression of these genes changed in a similar pattern in the two cultivars after TMV inoculation in both + S and-S treatments,and their expression in-S treatment was slightly lower than in + S treatment. The expression of the four genes related to TMV resistance( GST,PR1-a,HSP90 and Catalase-3) was up-regulated,and was higher in Yuyan 8 than in NC89 after TMV inoculation. On the contrast,the expression of the two genes involved in photosynthesis( PsbA and Photosystem Ⅱ10 kD a polypeptide) were down-regulated,and the decrease in Yuyan 8 was less than in NC89. The results indicate that in the cultivars resistant to TMV infection,enhanced glutathione metabolism is beneficial to cellular redox balance,and the stable expression of PsbA which encodes the PSⅡ reaction center protein D1 can reduce the damage to photosynthetic system.
This study was conducted to explore the relationship between glutathione metabolism and tobacco mosaic virus (TMV) resistance, using a TMV resistant tobacco cultivar Yuyan 8 and another tobacco cultivar NC89 which has the same genetic background with Yuyan 8 as the experimental materials. Bioinformatics analysis on the transcriptome data obtained from high-throughput sequencing revealed that among pathways enriched with differentially expressed genes, glutathione metabolic pathway was found in both cultivars infected with TMV, and glutathione metabolism was enhanced in Yuyan 8. We detected the changes in the transcription of glutathione S -transferase (GST) which is a key enzyme in glutathione metabolic pathway with quantitative PCR, the enzyme activity of GST and the content of glutathione (GSH) with spectrophotometry, and the expression levels of the genes that are related to TMV resistance or involved in photosynthesis. The results showed that GST gene expression, enzyme activity and GSH c ontent were all increased by TMV infection in both cultivars, and the increments in Yuyan 8 were more significant. The expression of GST and other genes relate to TMV resistance were verified under different sulfur conditions. a similar pattern in the two cultivars after TMV inoculation in both + S and-S treatments, and their expression in-S treatment was slightly lower than in + S treatment. The expression of the four genes related to TMV resistance (GST, PR1- a, HSP90 and Catalase-3) was up-regulated, and was higher in Yuyan 8 than in NC89 after TMV inoculation. On the contrast, the expression of the two genes involved in photosynthesis (PsbA and Photosystem II 10 kD a polypeptide) were down -regulated, and the decrease in Yuyan 8 was less than in NC89. The results that that in the cultivars resistant to TMV infection, enhanced glutathione metabolism is beneficial to cellular redox balance, and the stable expression of PsbA wh ich encodes the PSII reaction center protein D1 can reduce the damage to photosynthetic system.