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目的:观察外源性醛固酮(aldosteronre,Ald)灌注对大鼠心肌重构和氧化应激水平的影响,以及抗氧化剂维生素E(VitE)的干预作用。方法:大鼠随机分为:①对照组,②Ald组,③Ald+VitE组和④Ald+螺内酯(spironolactone,Spi)组。用黄嘌呤氧化法测定超氧化物歧化酶的活性,用巴比妥酸比色法测定丙二醛的含量,Masson三色法检测心肌胶原,细胞凋亡原位检测法检测心肌细胞凋亡。结果:与Ald组比较,Ald+VitE组心肌胶原、凋亡指数、丙二醛明显减少,超氧化物歧化酶明显增加(P<0.05)。Ald+VitE组与Ald+Spi组上述指标差异无统计学意义。结论:VitE能够降低Ald诱导的大鼠心肌氧化应激水平,显著改善心肌纤维化和心肌细胞凋亡。
AIM: To observe the effect of aldosteron (Ald) perfusion on cardiac remodeling and oxidative stress in rats and the effect of vitamin E (VitE). Methods: The rats were randomly divided into: ① control group, ② Ald group, ③ Ald + VitE group and ④ Ald + spiron group. The activity of superoxide dismutase (SOD) was measured by xanthine oxidation method. The content of malondialdehyde (MDA) was determined by barbituric acid colorimetric method. The myocardial collagen was detected by Masson’s trichrome method. The apoptosis of cardiomyocytes was detected by in situ cell apoptosis assay. Results: Compared with Ald group, collagen, apoptosis index and malondialdehyde in Ald + VitE group were significantly decreased and superoxide dismutase was significantly increased (P <0.05). Ald + VitE group and Ald + Spi group the above indicators no significant difference. Conclusion: VitE can reduce the level of oxidative stress induced by Ald in rats and significantly improve myocardial fibrosis and cardiomyocyte apoptosis.