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用荧光染料Indo-1标记小鼠腹腔巨噬细胞内游离钙,在粘附式细胞仪检测下观察细胞内游离钙的动态变化，在大肠杆菌O55：B5内毒素刺激下细胞内游离钙迅速升高，于第400s达峰值．经虎杖甙处理的巨噬细胞加入内毒素后，胞质游离钙无明显增高．因细胞浴于无钙环境，故胞内游离钙升高不是由于细胞外的Ca2+进入细胞，而是由于内毒素影响胞内钙库调节功能．虎杖甙可能保护胞内钙库中胞质钙的某种调节机制，从而避免胞质游离钙升高．
Intracellular free calcium in mouse peritoneal macrophages was labeled with the fluorescent dye Indo-1, and the dynamic changes of intracellular free calcium were observed under adherent cytometry, and the intracellular free calcium rapidly increased under the stimulation of Escherichia coli O55:B5 endotoxin. High, reaching the peak in the 400s. After addition of endotoxin to macrophages treated with Polygonum cuspidatum, cytoplasmic free calcium did not increase significantly. Because the cell bath is in a calcium-free environment, the increase of intracellular free calcium is not due to extracellular Ca2+ entry into the cell, but due to endotoxin affecting the regulation of intracellular calcium stores. Polygonum cuspidatum may protect a certain regulatory mechanism of cytosolic calcium in intracellular calcium stores, thereby avoiding the increase of cytosolic free calcium.