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目的探讨125Ⅰ放射性粒子植入对兔移植性肝癌微血管密度(microvascular density,MVD)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,MVD和VEGF的相关性及其与影像和临床病理的关系,评价125Ⅰ放射性粒子植入对兔VX2肝移植瘤血管生成及其疗效的影响,为原发性肝癌的放射性粒子植入治疗提供实验数据及理论依据。方法将36只荷瘤兔随机分成6组:对照组6只、粒子组30只。分别于接种后1周、2周、1月、2月、3月取材。测量肿瘤体积并行HE染色进行病理学检查,免疫组化染色检测肿瘤VEGF、CD31的表达。结果对照组肿瘤体积较前增大,粒子组肿瘤体积明显缩小。两组间相互比较粒子组与对照组之间均有明显的差异性(P<0.01);粒子组VEGF、MVD明显低于对照组(P<0.05),植入粒子不同时期VEGF、MVD有明显的差异(P<0.05)。结论 125I放射性粒子植入可以明显降低肿瘤VEGF、MVD的表达,可以有效地破坏植入粒子边缘肿瘤组织的微血管,有效地抑制血管形成并减少肿瘤组织的血液供应,从而达到灭活植入粒子边缘肿瘤组织的目的 。
Objective To investigate the effect of radioactive seed 125 I implantation on the expression of microvascular density (MVD) and vascular endothelial growth factor (VEGF) in transplantable hepatocellular carcinoma (HCC) in rabbits. The correlation between MVD and VEGF and its correlation with the imaging and clinicopathology To evaluate the effect of 125I radioactive seed implantation on the angiogenesis and its curative effect in VX2 liver xenograft in rabbits and to provide experimental data and theoretical basis for the treatment of primary liver cancer with radioactive particle implantation. Methods Twenty-six tumor-bearing rabbits were randomly divided into 6 groups: control group (6) and particle group (30). One week, two weeks, one month, two months and three months after inoculation respectively. The tumor volume was measured and pathological examination was performed by HE staining. The expression of VEGF and CD31 was detected by immunohistochemistry. Results The tumor volume in the control group increased compared with the previous group, and the tumor volume in the particle group was significantly reduced. There was significant difference between the two groups (P <0.01). The levels of VEGF and MVD in the particle group were significantly lower than those in the control group (P <0.05) (P <0.05). Conclusion 125I radioactive seed implantation can significantly reduce the expression of VEGF and MVD in tumor, which can effectively destroy the microvascular in the marginal tumor tissue, effectively inhibit the blood vessel formation and reduce the blood supply to the tumor tissue, so as to reach the edge of inactivated particle implantation The purpose of tumor tissue.