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心肌损害是阿霉素的主要副作用。我们分别采用体外心肌细胞培养和在体动物模型,观察门冬氨酸钾镁对阿霉素性心肌损伤的影响。以2～3天龄Wistas大鼠的心肌细胞为原料,取培养三天的心肌细胞19瓶,随机分成阿霉素组(n=10)和门冬氨酸钾镁加阿霉素组(n=9)(门冬氨酸钾镁系上海海普药厂产品,含钾10mg/ml,镁4mg/ml)。经阿霉素1mg/L、40mg/L的无血清含药培养基培养4小时后,用日立A220型双光束紫外分光光度计测定二组培养基中反映心肌细胞损伤程
Myocardial damage is a major side effect of doxorubicin. We used in vitro cardiomyocyte culture and in vivo animal models to observe the effects of potassium and magnesium aspartate on doxorubicin-induced myocardial injury. Nineteen-week-old cardiomyocytes were isolated from cardiomyocytes of 2 to 3-day-old Wistas rats and randomly divided into doxorubicin group (n = 10) and potassium-magnesium aspartate and adriamycin group = 9) (potassium magnesium aspartate Shanghai Haipu pharmaceutical products, potassium 10mg / ml, magnesium 4mg / ml). After adriamycin 1mg / L, 40mg / L serum-free drug-containing medium for 4 hours, with Hitachi A220 dual-beam UV spectrophotometer to measure the two groups of media reflect the damage of myocardial cells