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目的研究颊黏膜鳞癌组织中促凋亡因子蛋白激酶C-θ(PKC-θ)的表达及临床意义。方法应用SABC免疫组化法检测PKC-θ蛋白在36例颊黏膜鳞癌组织中的表达,以13例正常颊黏膜上皮组织作为对照。颊黏膜鳞癌标本经10%甲醛固定,石蜡包埋,按高、中、低分化进行病理学分级。结果 36例颊黏膜鳞癌组织中PKC-θ阳性率仅为30.6%,13例对照组颊黏膜上皮细胞中PKC-θ阳性率100%。正常颊黏膜组织中上皮细胞与颊黏膜鳞癌组织PKC-θ阳性率差异有统计学意义(P<0.01);颊黏膜鳞癌中PKC-θ表达与病理分级无关(P>0.05)。结论颊黏膜鳞癌组织中PKC-θ存在缺失或低表达。
Objective To study the expression of PKC-θ and its clinical significance in buccal mucosal squamous cell carcinoma. Methods The expression of PKC-θ protein in 36 cases of buccal mucosal squamous cell carcinoma was detected by SABC immunohistochemical method, and 13 cases of normal buccal mucosa epithelial tissue were taken as control. Buccal mucosal squamous cell carcinoma specimens were fixed with 10% formaldehyde and embedded in paraffin, pathologically graded according to high, medium and low differentiation. Results The positive rate of PKC-θ in 36 cases of buccal squamous cell carcinoma was only 30.6%. The positive rate of PKC-θ in the buccal epithelial cells of 13 cases was 100%. The positive rate of PKC-θ in normal buccal mucosa epithelial cells and buccal mucosa squamous cell carcinoma tissues had statistical significance (P <0.01). The expression of PKC-θ in buccal mucosa squamous cell carcinoma had no correlation with pathological grade (P> 0.05). Conclusion PKC-θ in the buccal mucosal squamous cell carcinoma is missing or low expression.