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用荧光加Giemsa(FPG)术区分姐妹染色单体,分析了13名中年男子外周血淋巴细胞的SCE,比较吸烟者和不吸烟者“自发”的和用丝裂霉素(MMC)诱发的SCE频率变化。发现吸烟者的SCE频率(8.33±1.08)显著地高于不吸烟者(4.41±0.72),p<0.001。用MMC诱发后吸烟者各剂量的SCE值也明显地高于不吸烟者(P<0.01),并随着MMC剂量的增加,两者之间的差值增大。由于SCE的形成同DNA损伤后的修复机制有关,SCE频率的不同反映交换产生过程中机体重组修复系统的差异。因此,吸烟者与不吸烟者SCE频率的差异,表明两者在DNA修复机制上存在着差异。吸烟很可能改变了机体DNA损伤后的修复能力。
SCE was analyzed by fluorescence plus Giemsa (FPG) technique in peripheral blood lymphocytes from 13 middle-aged men, compared with “spontaneous” and mitomycin (MMC) -induced smokers and non-smokers SCE frequency changes. The frequency of SCE was found to be significantly higher in smokers (8.33 ± 1.08) than in nonsmokers (4.41 ± 0.72), p <0.001. The SCE of each dose of smoker induced by MMC was also significantly higher than that of non-smoker (P <0.01), and with the increase of MMC dose, the difference between them increased. Because the formation of SCE is related to the repair mechanism of DNA damage, the difference of SCE frequency reflects the difference of the system of recombination and repair in the process of exchange. Therefore, the differences in SCE frequency between smokers and non-smokers suggest that there is a difference in the mechanism of DNA repair between the two. Smoking is likely to change the body’s ability to repair DNA damage.