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1977年,当Rizzetto及其同事在意大利Turin用直接免疫荧光法,研究乙型肝炎病毒在HBsAg阳性病人肝活切片中分布时才发现delta因子。他们注意到在他们所用的试剂中有一种荧光素结合人抗-HBc丙种球蛋白片段的试剂,能与电镜下证实不含核心颗粒的肝活体切片产生核内荧光。这些切片与其他参考抗-HBc血清不起反应,但与其不含抗-HBc的某些HBsAg阳性血清显示核内荧光。因为由这些抗核心阴性血清所显示的荧光只能从HBsAg阳性病人的标本中出现,从阻抑和吸收试验也明确表明这不是由于非
In 1977, when Rizzetto and colleagues in Turin, Italy, used direct immunofluorescence to study the distribution of hepatitis B virus in liver biopsies from HBsAg-positive patients, the delta factor was found. They noticed that there was a reagent that fluorescein bound human anti-HBc gamma globulin among the reagents they used that could produce intranuclear fluorescence with electron microscopy to confirm that the liver biopsy without core particles produced nuclear localization. These sections did not react with the other reference anti-HBc sera, but showed some nuclear fluorescence with some of the HBsAg positive sera without anti-HBc. Because the fluorescence shown by these anti-core negative sera appears only in specimens from HBsAg-positive patients, it is also clear from the suppression and uptake tests that this is not due to non-