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采用ＧＣ－ＭＳ等方法证实了硝基安定热裂解的主要产物之一是２－氨基－５－硝基苯基苯甲酮，并建立了热裂解薄层色谱法。首次采用重氮偶合显色在薄层板上定量测定硝基安定。实验条件为样品过ＸＡＤ－２柱，乙酸乙酯洗脱，进一步处理后进行ＴＬＣ，然后热裂解。重氮偶合显色后，ＣＳ－９３０薄层扫描仪测定，波长５３５ｎｍ。反射吸收法，锯齿扫描，以硝基安定与内标的峰面积比，与标准值比较定量，血浆样品线性范围０．１５～１５μｇ／ｍｌ，最低检出量０．１μｇ／ｍｌ。
Using GC-MS and other methods confirmed nitrobenzene thermal cracking of one of the main products is 2-amino-5-nitrophenyl benzophenone, and the establishment of the thermal cracking thin layer chromatography. For the first time, diazotized coupling color was used to quantitatively measure nitrazepam on the TLC plate. The experimental conditions for the sample over XAD-2 column, ethyl acetate, further processing after TLC, and then pyrolysis. Diazo coupling color, CS-930 TLC scanner, wavelength 535nm. Reflectance absorption method, sawtooth scan to nitrazepam and the internal standard peak area ratio, compared with the standard quantitative, linearity of plasma samples 0.15 ~ 15μg / ml, the minimum detection amount of 0.1μg / ml.