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在基因枪介导转化的转基因水稻植株中发现1个四倍体变异株系XIP-4N.该变异株系T0代植株中转基因的整合模式与二倍体转基因株系XIP-2N相同,并且二者来自同一转化体系,推测XIP-4N株系是转化后的水稻愈伤组织在细胞有丝分裂过程中发生染色体加倍产生的.对外源筛选标记基因bar和非筛选基因cecropinB在转基因株系XIP-4N和XIP-2N中的遗传行为进行了比较研究.在二倍体转基因株系XIP-2N中,bar和cecropinB基因的Southern整合模式从T0到T2代遗传稳定,单位点整合的bar基因按孟德尔单基因显性方式向后代传递.四倍体转基因株系XIP-4N中外源基因遗传行为复杂,单位点整合的bar基因(Basta抗性)T1代按15∶1分离,T2和T3代中分离行为复杂,而且bar和cecropinB基因的整合模式遗传不稳定.同源四倍体水稻植株减数分裂过程中染色体结构变异、转基因相关位点DNA片段的遗传重组与修饰,以及由此导致配子的育性降低,可能是导致外源基因遗传行为复杂的主要原因.转基因四倍体水稻变异株系XIP-4N携带易于检测的bar基因,为研究同源四倍体水稻的遗传和生殖机理提供了好材料.
One tetraploid mutant XIP-4N was found in the transgenic rice-mediated transgenic rice plants.The integration pattern of the transgene in the T0 generation was the same as that of the diploid transgenic strain XIP-2N, Derived from the same transformation system and speculated that the XIP-4N strain is produced by doubled chromosomal duplication in the transformed rice callus during cell mitosis.Expression of exogenous screening marker bar and non-screening gene cecropinB in transgenic lines XIP-4N and XIP-2N.The Southern integration pattern of bar and cecropinB gene was stable from T0 to T2 generation in the diploid transgenic line XIP-2N.The single-point integrated bar gene was analyzed by Mendelian single Gene transfer to the offspring.The inheritance of foreign genes in tetraploid transgenic lines XIP-4N was complex, the single-point integrated bar gene (Basta resistance) T1 generation was segregated at 15: 1 and the segregation behavior at T2 and T3 Complex, and the integrated mode of bar and cecropinB genes is not stable.Chromosome structural variation during meiosis of autotetraploid rice plants, genetic recombination and modification of DNA fragments of transgenic-related sites, and thus The reduced fertility of the gametophyte may be the main reason leading to the complex genetic behavior of the exogenous gene.The transgenic tetraploid rice XIP-4N carries a readily detectable bar gene. In order to study the genetic and reproductive activities of autotetraploid rice, Mechanism provides good material.