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将硅烷偶联剂γ-(2,3-环氧丙氧)丙基三甲氧基硅烷(KH560)先与十八醇反应,再键合到硅胶整体柱上,得到醚型十八烷基键合硅胶整体柱,并用红外光谱对其进行了表征。利用该固定相,在甲醇-水简单的二元流动相体系下,考察了该整体柱适用的pH范围,以及流速与柱压降和流速与柱效的关系,用该固定相对苯及其同系物进行了分离,并对尼群地平、硝苯地平和间尼索地平进行了分离。结果表明,该硅胶整体柱键合效果良好,具有较好的反相色谱性能,且在pH=2~8范围内稳定性好,柱压降、柱效受流速影响较小,可有效地用于化合物的快速分离分析。
The silane coupling agent γ- (2,3-epoxypropoxy) propyltrimethoxysilane (KH560) first with octadecanol, and then bonded to the silica gel monolithic column to give ether type octadecyl bond The silica gel monolithic column was characterized by FTIR. Using this stationary phase, the suitable pH range of the monolithic column, the relationship between the flow rate, the column pressure drop, the flow rate and the column efficiency were investigated under the simple binary mobile phase of methanol-water system. With this stationary phase, The substances were separated and nitrendipine, nifedipine and nisoldipine were separated. The results showed that the silica gel monolithic column bonding effect is good, with good reverse phase chromatographic performance, and in the pH = 2 ~ 8 range of good stability, column pressure drop, column efficiency less affected by the flow rate can be effectively used Rapid separation and analysis of compounds.