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目的 研究 p16基因在胃癌组织中的表达及其与胃癌生物学行为的关系。 方法 采用免疫组化和原位杂交方法检测 10例正常胃粘膜、30例胃癌组织中 p16基因mRNA和蛋白的表达 ,并结合其临床资料进行分析。结果 p16基因mRNA和蛋白阳性表达率 ,在正常胃粘膜均为90 % ,而胃癌分别为 43 33%和 36 6 7%。胃癌组与正常对照组间差异均有显著意义 (P <0 0 5 ) ,p16基因mRNA和蛋白与胃癌分化程度、淋巴结转移与否、远处转移与否呈一定的相关性。结论 p16基因与胃癌发生有关 ,其表达产物检测可能有助于胃癌预后的判断。
Objective To study the expression of p16 gene in gastric carcinoma and its relationship with the biological behavior of gastric cancer. Methods Immunohistochemistry and in situ hybridization were used to detect the expression of p16 mRNA and protein in 10 cases of normal gastric mucosa and 30 cases of gastric carcinoma, and their clinical data were analyzed. Results The positive rates of p16 gene mRNA and protein were 90% in normal gastric mucosa and 43.33% and 36.67% in gastric cancer respectively. The difference between gastric cancer group and normal control group was significant (P <0.05). The mRNA and protein of p16 gene were correlated with the differentiation of gastric cancer, lymph node metastasis and distant metastasis. Conclusion The p16 gene is associated with the occurrence of gastric cancer, and the detection of its expression product may be helpful to the prognosis of gastric cancer.