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选用金黄色葡萄球菌、大肠杆菌及变形杆菌敏感株,经药物梯度平皿筛选得到耐药性突变株,测定细菌生长浊度变化。以利福定(RFD)或EDTA分别作用时,耐药菌的生长曲线不受影响,RFD和EDTA合用,则耐药菌的生长曲线受到明显抑制。以~3H-RFD分别掺入敏感菌及耐药菌,前者的药物掺入量明显高于后者。电镜观察见耐药菌菌体变得粗短,表面粗糙,外膜增厚,膜皱折增多。以上结果提示,细菌包膜屏障在耐药性产生中具有重要作用。
Staphylococcus aureus, Escherichia coli and Proteus sensitive strains were selected and drug-resistant mutant strains were screened by drug gradient plate to determine the change of bacterial growth turbidity. The growth curves of drug-resistant bacteria were not affected when using RFD or EDTA respectively. The combination of RFD and EDTA significantly inhibited the growth of resistant bacteria. With ~ 3H-RFD were sensitive bacteria and resistant bacteria, the former drug incorporation was significantly higher than the latter. Electron microscopy showed resistant bacterial cells become stubby, rough surface, thickening of the outer membrane, the membrane wrinkles increased. The above results suggest that the bacterial envelope barrier plays an important role in drug resistance.