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本文对采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）对梭形住肉孢子虫和巨型住肉孢子虫的包囊壁（ＣＷ）、包囊液（ＣＦ）与缓殖子（ＣＴ）三部分的可溶性蛋白进行了分析，从各组份区分出１０—２６条蛋白带，分子量９—１００ｋＤａ，ＣＷ以高分子量蛋白为主，ＣＦ则以５０ｋＤａ以下低分子量蛋白带居多，ＣＴ各种蛋白带分布较均匀。应用超凝胶ＡＣＡ５４柱层析和ＳＤＳ—ＰＡＧＥ对梭形住肉孢子虫包囊蛋白进行了分离纯化，分离出７２ｋＤａ蛋白，经免疫印渍术鉴定为单特异性抗原、经对８６头水牛的ＥＬＩＳＡ检疫分析，其纯化抗原较未纯化的粗抗原，特异性增强，但敏感性降低。
In this paper, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on the spindle-shaped Sarcocystis carnosus and giant Sarcocystis cyst wall (CW), cyst fluid (CF) (CT) three parts of the soluble protein were analyzed from the components of the distinction of 10-26 bands with a molecular weight of 9-100kDa, CW to high molecular weight protein, CF 50kDa below 50kDa mostly low molecular weight protein band, CT protein bands more evenly distributed. The cytoplasm of Cytospora sp. Was separated and purified by supercritical ACA54 column chromatography and SDS-PAGE. The 72 kDa protein was isolated and identified as monospecific antigen by immunoblotting. ELISA quarantine analysis, the purified antigen crude antigen than the unpurified, increased specificity, but reduced sensitivity.