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AIM:To evaluate the effect of mitochondrial tumor necrosis factor receptor-associated protein-1(TRAP-1) on the lymph node metastasis(LNM) in Chinese colorectal cancer(CRC) patients,and develop potential LNMassociated biomarkers for CRC using quantitative realtime polymerase chain reaction(RT-PCR) analysis.METHODS:Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM(LNM CRC) and without LNM(non-LNM CRC) were assessed in 96 Chinese colorectal carcinoma samples using quantitative RT-PCR analysis,Western blotting,and confirmed with immunohistochemical assay.The relationship between clinicopathological parameters and potential diagnostic biomarkers was also examined.RESULTS:TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC,which was confirmed by RT-PCR,Western blotting and immunohistochemical assay.The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines,LoVo and HT29,was analyzed with Western blotting.The expression level of TRAP-1 was dramatically higher in LoVo than in HT29.Overexpression of TRAP-1 was significantly associated with LNM(90.2% in LNM group vs 22% in non-LNM group,P < 0.001),the advanced tumor node metastasis stage(89.1% in LNM group vs 26.9% in non-LNM group,P < 0.001),the increased 5-year recurrence rate(82.7% in LNM group vs 22.6% in non-LNM group,P < 0.001) and the decreased 5-year overall survival rate(48.4% in LNM vs 83.2% in non-LNM group,P < 0.001).Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients(Hazard ratio of 2.445 in recurrence,P = 0.017;2.867 in survival,P = 0.028).CONCLUSION:Mitochondria TRAP-1 affects the lymph node metastasis in CRC,and may be a potential biomarker for LNM and a prognostic factor in CRC.Overexpression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients.
AIM: To evaluate the effect of mitochondrial tumor necrosis factor receptor-associated protein-1 (TRAP-1) on the lymph node metastasis (LNM) in Chinese colorectal cancer (CRC) patients, and develop potential LNMassociated biomarkers for CRC using quantitative realtime polymerase (RT-PCR) analysis.METHODS: Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed in 96 Chinese colorectal carcinoma samples using quantitative RT- PCR analysis, Western blotting, and confirmed with immunohistochemical assay. and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diagnostic biomarkers was also examined .RESULTS: TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immunohistochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blott ing.The expression level of TRAP-1 was higher higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM (90.2% in LNM group vs 22% in non-LNM group, P <0.001), the advanced tumor node metastasis stage (89.1% in LNM group vs 26.9% in non-LNM group, P <0.001), the increased 5-year recurrence rate (82.7% in LNM group vs 22.6% in non-LNM group, P <0.001) and the decreased 5-year overall survival rate (48.4% in LNM vs. 83.2% in non-LNM group, P <0.001) .Univariate and multivariate analyzes showed that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients (Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028) .CONCLUSION: Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential biomarker for LNM and a prognostic factor in CRC. Overexpression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients.