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目前已能在一定的化学琼脂培养基上培养若干种作物未成熟的花药,以产生大量单倍体植株。这里所介绍的资料说明,培养基组成、提供花药植株所处的环境条件、花药培养的条件、小孢子的细胞学阶段和培养物种的基因型,对采用花药培养成功产生单倍体植株和获得其它各种植物单倍体植株的尝试都有影响。目前已有一些方法正成功地用于单倍体植株染色体数的加倍。从这些单倍体植株诱导的单倍体植株和纯合的双倍体正在应用的和基础的研究中被采用。由于单倍体技术的应用,使化学和农艺性状被改变的烟草属优良选系得到发展。单倍体植株材料在诱发突变形成的研究中特剐有用。花药培养业已成为发展烟草零式单倍体(nullihaploid)、缺对染色体、三体及四体等非整倍体的重要手段。
It is now possible to cultivate several kinds of immature anther on a certain chemical agar medium to produce a large number of haploid plants. The data presented here describe the composition of the medium, the environmental conditions in which the anther plants are located, the conditions of the anther culture, the cytological stages of the microspores and the genotypes of the cultivated species. Successful production of haploid plants using anther culture and obtaining Other attempts to plant haploid plants have implications. At present, some methods are successfully used to double the chromosome number of haploid plants. Haploid plants induced from these haploid plants and homozygous diploids are being used in and basic studies. Due to the application of haploid technology, the development of fine lineages of tobacco with altered chemical and agronomic traits has been developed. Haploid plant material is particularly useful in studies that induce the formation of mutations. Anther culture has become an important tool for the development of aneuploidy such as nullihaploid, absent chromosomes, trisomy and tetramer.