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以往栽培黑木耳一般都使用木屑和木粒菌种,而纯木屑不宜得到、木粒种工本过高,为此我们进行了用小麦粒生产黑木耳菌种的可行性研究,取得了满意效果。一、原种制作取干净的小麦粒直接放入锅中加水煮沸约半小时(煮至胀饱为宜),取出沥干表面水份后拌入麦粒干重2％的石膏粉,装入小口径瓶中(每瓶约装干小麦150——200克),塞好棉塞放入常压灭菌锅100℃处理10小时,冷却后取出摇匀即可接种。同时以常规木屑麸皮培养基作比较。每支180×18mm的斜面试管母种可接2——6瓶原种。接种后置25——32℃下培养,麦粒种20多天、木屑种30多天即可全部满瓶。二、原种扩接栽培种在无菌条件下把长满菌丝的麦粒原种掏散以后,每瓶栽培种
In the past, the cultivation of black fungus generally used sawdust and wood species, and pure wood chips should not be obtained, the wood seedlings are too high, for which we carried out the feasibility of using black wheat ear fungus species, and achieved satisfactory results. First, the original production Take clean wheat grains directly into the pot and add water to boil for about half an hour (cook to sweat full advisable), remove the surface moisture after mixing dry grit 2% of gypsum powder, into Small-caliber bottles (each bottle of about 150--200 grams of dry wheat), stuffed tampon into an autoclave 100 ℃ for 10 hours, remove the shake after cooling can be inoculated. At the same time compared with conventional sawdust bran medium. Each 180 × 18mm incline test tube mother can be accessed 2--6 bottles of original species. After inoculation 25--32 ° C under cultivation, more than 20 kinds of wheat seed, wood species for more than 30 days can be full of bottles. Second, the original expansion of cultivated species In sterile conditions to overgrow the original varieties of wheat mycelium, after each bottle of cultivated species