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目的分析弱D15型个体的红细胞膜表面的D抗原的表位情况。方法采用间接抗球蛋白试验通过12种抗D抗原不同表位的人抗-D单克隆抗体,检测2名通过基因测序确认的已知弱D15型个体的红细胞膜D抗原表位,分别以Rh阳性、Rh阴性作为对照。结果弱D15型个体的红细胞膜D抗原12个抗原表位中有7种检测为阳性,5种检测为阴性。结论弱D15型个体红细胞膜D抗原除分子数量减少外,还存在“质”的变化,提示其可能具有“部分D”的抗原特性。
Objective To analyze the epitopes of D antigen on the surface of erythrocyte membrane in individuals with weak D15. Methods The indirect antiglobulin test was used to detect the epitope of erythrocyte D antigen in two weakly known D15 individuals identified by gene sequencing using 12 anti-D monoclonal antibodies against different epitopes of D antigen. Positive, Rh negative as a control. Results Weak D15 type of individual erythrocyte membrane antigen D antigen 12 epitopes were detected as positive 7, 5 were negative. Conclusion In addition to the decrease of the molecular weight of D-type Erythrocyte Membrane D-antigen, the D-type Erythrocyte Membrane D antigen also has the change of “quality”, suggesting that it may have the antigenic characteristic of “partial D”.