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目的探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)分化的作用。方法根据文献从成年大鼠海马原代培养NG2细胞,7天后添加10μl/ml(0.4mg/ml)CL继续培养7天,以免疫荧光双重染色法鉴定细胞性质。结果10μl/mlCL处置明显增加NG2细胞中Map2a/b、SynapsinI、VGAD和GABA表达水平。结论CL促进NG2细胞向神经元(特别是GABA能抑制性中间神经元)分化。
Objective To investigate the effect of cerebrolysin (CL) on the differentiation of NG2 proteoglycan positive neural progenitor cells (NG2 cells). Methods NG2 cells were primarily cultured from the hippocampus of adult rats according to the literature. After 7 days, 10 μl / ml (0.4 mg / ml) CL was added to continue the culture for 7 days. The cell characteristics were identified by double immunofluorescence staining. Results 10μl / mlCL treatment significantly increased the expression of Map2a / b, Synapsin I, VGAD and GABA in NG2 cells. Conclusion CL promotes the differentiation of NG2 cells into neurons (especially GABA-inhibitory interneurons).