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目的:分析红曲霉与何首乌双向液体发酵形成的药性基质主要活性成分,优选双向发酵工艺条件。方法:采用紫外-可见全波长扫描法和紫外分光光度法进行定性和定量分析,以二苯乙烯苷、结合蒽醌、游离蒽醌和莫纳可林K含量为评价指标,在单因素试验基础上,通过正交试验考察红曲霉接种量、何首乌添加量和发酵时间对红曲霉与何首乌双向液体发酵工艺的影响。结果:基质中二苯乙烯苷、结合蒽醌和总蒽醌含量呈下降趋势,而游离蒽醌含量上升,双向发酵最佳工艺条件为何首乌用量5%,发酵温度28℃,接种量6%,发酵时间7 d。二苯乙烯苷、结合蒽醌、游离蒽醌、莫纳科林K质量分数分别为37.84,0.89,1.54,0.41 mg·g-1。结论:何首乌对红曲霉的生长及其次级代谢产物莫纳科林K的积累具有促进作用,红曲霉降低了何首乌中结合蒽醌的含量,提高了何首乌的药用价值。
OBJECTIVE: To analyze the main active ingredients of drug-based matrix formed by bi-directional liquid fermentation of Monascus and Polygonum multiflorum, and optimize bi-directional fermentation conditions. Methods: Qualitative and quantitative analysis was carried out by ultraviolet-visible full-wavelength scanning and ultraviolet spectrophotometry. The contents of stilbene glycoside, anthraquinone, free anthraquinone and monacolin K were used as evaluation indexes. Based on the single factor test The effects of inoculum size, Monascus polygoni multiflori addition and fermentation time on the bi-directional liquid fermentation of Monascus and Polygonum multiflorum were investigated by orthogonal test. Results: The contents of stilbene glycoside, anthraquinone and total anthraquinone in the matrix decreased while the content of free anthraquinone increased. The optimum conditions for bi-directional fermentation were Radix Polygoni multiflori (Radix Polygoni multiflori) in 5%, fermentation temperature 28 ℃, inoculum 6% Fermentation time 7 d. Stilbene, anthraquinone, free anthraquinone, monacolin K were 37.84,0.89,1.54,0.41 mg · g-1. Conclusion: Polygonum multiflorum plays an important role in the growth of monascus and the accumulation of monacrine K in monascus. Monascus decreases the anthraquinone content in the polygonum multiflorum and increases the medicinal value of Polygonum multiflorum.