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本文报道了用细胞松驰素B、高温和低温休克等方法诱导华贵栉孔扇贝（Chloamysnobilis）三倍体的实验结果。在26.0～28．0℃条件下，选用不同浓度的细胞松驰素B0．1、0．3、0．5、0．7和1．0mg／dm3，在受精后15min，处理受精卵15min，三倍体诱导率分别为12．5％、37．5％、68．0％、66．6％和81．0％，其中0．5～1．0mg／dm3浓度的细胞松驰素B对改变倍性最有效。高温休克选用31．0、33.0、35.0和37．0℃处理受精卵，最高三倍体诱导率达46.7％（33．0℃，处理15min）。低温休克选用6．0、8．0、10．0和12．0℃处理受精卵，最高三倍作诱导率为40．7％（10．0℃，处理20min）。实验结果表明，随着处理强度的增大和处理时间的延长，三倍体诱导率增高、但孵化率下降，畸型率上升。用高温休克处理的实验组幼虫生长速度较对照组快。作者认为，在商业性生产中，温度休克是诱导扇贝三倍体的一种简便、有效的方法。文中还就细胞松驰素B与温度休克诱导三倍体的机制进行了讨论。
This paper reports the experimental results of the induction of triploid of Chloamysnobilis with cytochalasin B, high temperature and low temperature shock. Under the conditions of 26.0 ~ 28.0 ℃, different concentrations of cytochalasin B0.1,0.3,0.5,0.7 and 1.0mg / dm3 were selected, 15min fertilization after 15min fertilization, The triploid induction rate was 12.5%, 37.5%, 68.0%, 66.6% and 81.0%, respectively. The cytochalasin B concentration of 0.5 ~ 1.0mg / dm3 Change ploidy most effectively. High temperature shock selected 31.0,33.0,35.0 and 37.0 ℃ fertilized eggs, the highest triploid induction rate of 46.7% (33.0 ℃, 15min). Cryopreservation shock eggs were treated with 6.0, 8.0, 10.0 and 12.0 ℃, the highest induction rate was 40.7% (10.0 ℃, 20min). The experimental results showed that with the increase of treatment intensity and the extension of treatment time, the induction rate of triploid increased but the hatching rate decreased and the rate of abnormality increased. The experimental group treated with heat shock larvae grew faster than the control group. The authors believe that in commercial production, temperature shock is a simple and effective method to induce triploid trichomes. The mechanism of cytokinin B and triploid induction by temperature shock was also discussed.