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目的探讨TRB3基因+251A/G多态性与代谢综合征(MS)患者左房功能改变的相关性。方法选择MS患者177例,正常对照组156例。采用酚/氯仿法提取全血DNA,PCR-RFLP法检验受试者TRB3基因第二外显子+251A/G的多态性位点。应用应变率成像技术测量左房各壁的应变率。结果 +251A/G位点AA、AG+GG基因型和等位基因的频率分布在正常对照组和MS组,两组间差异有统计学意义(P均<0.05);与正常对照组相比,MS组左房平均收缩期应变率峰值(mean SSR)、平均舒张早期应变率峰值(mean ESR)显著降低(P均<0.001),提示MS患者左房储存器和管道功能显著减低,左房平均舒张晚期应变率峰值(mean ASR)差异无统计学意义(P>0.05),MS患者左房助力泵功能无显著变化;与AA基因型相比,AG+GG基因型者左房mean SSR、mean ESR和mean ASR均明显降低(P均<0.05);多元线性回归分析显示mean ESR、mean ASR与TRB3基因+251A/G多态性G等位基因之间存在数量依存关系(P<0.05)。结论 TRB3基因+251A/G多态性G等位基因与MS患者左房功能受损相关,在MS患者左房功能损害中可能起重要作用。
Objective To investigate the association of TRB3 + 251A / G polymorphism with left atrial function in patients with metabolic syndrome. Methods 177 cases of MS patients were selected, 156 cases of normal control group. Whole blood DNA was extracted by phenol / chloroform method. The second exon + 251A / G polymorphism of TRB3 gene was tested by PCR-RFLP. Strain rate of left atrial wall was measured by strain rate imaging. Results The frequencies of genotypes and alleles of AA, AG + GG at + 251A / G locus were significantly different between the normal control group and the MS group (P <0.05). Compared with the normal control group , Mean left ventricular systolic strain mean (SSR) and mean early diastolic strain mean (ESR) decreased significantly in MS group (all P <0.001), suggesting that left atrial compartment and duct function were significantly reduced in MS patients, There was no significant difference in the mean ASR between the two groups (P> 0.05). There was no significant change in left atrial appendage pump function in MS patients. Compared with AA genotype, Mean ESR and mean ASR were significantly lower (P <0.05). Multivariate linear regression analysis showed that there was a quantitative relationship between mean ESR, mean ASR and G allele of TRB3 + 251A / G polymorphism (P <0.05) . Conclusion The G allele of TRB3 + 251A / G polymorphism is associated with impaired left atrial function in MS patients and may play an important role in the impairment of left atrial function in patients with MS.