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在长期夫妻生活中,生殖器官粘膜可能有多次细微的损伤,这会引起精子抗原的局部免疫。精子的穿透力可因宫颈粘液中抗精子抗体的存在或因精子负有抗精子抗体而被破坏。故在不孕症时,可用“性交后试验”()作免疫的定性检查。性交后试验:根据性交后妇女宫颈分泌物中活动的精子数确定不孕症时宫颈粘液内精子穿透力被破坏的程度。该试验在妇女排卵期进行,受检者于性交后6~10小时,采取宫颈粘液一滴置于已预热的玻片上,复以盖片,边缘以石腊封闭,立即镜检。在7倍目镜和40倍物镜下计算5个视野中的精子量。标本中可见向前运行的活精子、摆动的精子和不活动的精子。结果判断分五种类型:①为优等型:属正常。在5个视野中可见15~25个以上向前
In long-term couples, reproductive organ mucosa may have many subtle damage, which can cause local immunity of sperm antigens. Penetration of sperm may be due to the presence of anti-sperm antibodies in cervical mucus or due to sperm being rendered antisperm antibodies. Therefore, infertility, available “sexual intercourse test” () for qualitative immunological tests. Post-intercourse test: The degree of penetration of spermatozoa in cervical mucus is destroyed as infertility is determined by the number of sperm motile in cervical secretions of women after sexual intercourse. The test during ovulation in women, the subjects 6 to 10 hours after intercourse, take a drop of cervical mucus placed on a preheated slide, re-cover sheet, the edge of the paraffin closed, and immediately microscopic examination. The amount of sperm in five fields was calculated under 7 × eyepiece and 40 × objective. Specimens can be seen moving forward live sperm, swaying sperm and inactive sperm. Judgment results divided into five types: ① for the superior type: a normal. Visible in 5 fields 15 to 25 or more forward