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目的研究c(RGDfK)肽修饰后的纯钛表面对小鼠成骨细胞黏附、增殖的影响。方法应用超声微弧氧化技术和多巴胺化学偶联的方式分别在纯钛表面构建MAO-PDA-GRGDSP(X组)、MAO-PDA-c(RGDfK)(H组)和MAO(M组)功能涂层,采用扫描电镜进行形貌分析,通过CCK-8实验检测和激光共聚焦观察MC3t3-E1细胞在各组的早期黏附和增殖情况。结果扫描电镜检测涂层呈多孔形貌,多巴胺的修饰孔变小,并有颗粒状的RGD肽在微孔内。CCK-8结果显示,H组的OD值在各个时间点上都高于X组和M组,且各组比较具有统计学意义(P<0.05)。激光共聚焦观察细胞在X组和H组表面的状态均好于M组,但H组表面细胞的数目更多,丝足铺展的面积更大。结论线形肽GRGDSP和环形肽c(RGDfK)对细胞的早期黏附和增殖皆有促进作用,且环形肽c(RGDfK)的作用更强。
Objective To study the effect of c (RGDfK) peptide modified titanium surface on the adhesion and proliferation of mouse osteoblasts. Methods MAO-PDA-GRGDSP (group X), MAO-PDA-c (group RGDfK) (group H) and group MAO (group M) were applied on the surface of pure titanium by ultrasonic micro-arc oxidation and dopamine chemical coupling The morphology of the MC3t3-E1 cells was observed by scanning electron microscope (CCM-8), and the adhesion and proliferation of MC3t3-E1 cells in each group were observed by CCK-8 test and confocal laser scanning microscope. Results Scanning electron microscopy showed that the coating was porous and the dopamine modified pores were smaller, and the granular RGD peptide was in the pores. The results of CCK-8 showed that the OD value of H group was higher than that of X group and M group at each time point, and the difference between each group was statistically significant (P <0.05). Laser confocal microscopy showed that cells in group X and group H were better than group M, but the number of surface cells in group H was more and the area of silk foot spread was larger. Conclusions The linear peptides GRGDSP and RGDfK promote the early adhesion and proliferation of cells, and the effect of RGDfK is stronger.