The influence of sample volume applied to the Makler sperm counting chamber upon the measured concen

• 作 者: Melanie Walls Emily Zuvela Cheryl Ayres Deborah Sherrin Asma Chhotani Liz Butler Kelli Peirce Jenny Krapez Renae Parker Cherise Mooy Patrick Mohan Sally Catt Matthew Wiltshire Hassan W Bakos Mary Whyt
• 机 构: Fertility Specialists of WA, Australia“,”Demeter Laboratories, Australia“,”Queensland Fertility Grou
• 出 处: 亚太生殖杂志（英文版）
• 发表日期: 2012年1期
• 关键词: Volume Semen Makler chamber

Objective:To undertake a multi-centre study to maximize the number ofMakler chambers used.Methods:A total of15 laboratories participated with31Makler chambers.A suspension of latex beads was prepared to a concentration of20 millions per milliliter, and0.5 mL aliquots distributed to each participating laboratory.They measured the concentration on theirMakler chamber(s) used for routine semen analysis by adding3,4,5,7 and10μL volumes of bead suspension to the chamber.Results:There was no difference in within-chamber analysis of the bead concentration according to the volume of bead suspension applied within the range of 3-10μL(F4,14=2.634,P=0.056).However, the between-chamber effects were significantly different (F30,124=4.937,P=0.000), and24/31(77.5%) chambers tested had an average bias>10% compared to the target bead concentration.Conclusions:A volume of3-10μL added toMakler counting chambers does not influence the concentration measured of latex beads, but the between-chamber variability and positive bias seen would suggest that other sources of error are present which are yet to be identified.