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目的探讨P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP1)、肺耐药相关蛋白(LRP)和谷胱甘肽-S转移酶(GST-π)在中晚期喉鳞状细胞癌患者放疗前后肿瘤组织中表达及放疗对喉癌耐药基因的影响。方法收集76例中晚期喉癌患者,其中低分化鳞状细胞癌8例,中高分化鳞状细胞癌68例,采用免疫组织化学法检测在不同类型喉癌组织和放疗前后癌组织中P-gp、MRP1、LRP和GST-π的表达强度和分布,分析其表达情况。结果喉鳞状细胞癌具有原发耐药性,P-gp在低分化鳞癌和中高分化鳞癌2种病理类型喉癌组织中呈高强度强阳性表达,与MRP1、LRP和GST-π三种耐药蛋白比较差异有统计学意义(F=8.0839,P=0.0438);MRP1在低分化鳞癌中的表达明显高于中高分化鳞癌中的阳性表达率,其差异有统计学意义(t=9.575,P<0.001);放射治疗后P-gp、MRP1、LRP和GST-π阳性表达率与放射治疗前相比均有增高,其中P-gp在放疗前后表达阳性率分别为(53.26±7.94)%、(79.37±7.73)%,表达差异有统计学意义(t=6.28,P<0.05);MRP1在放疗前后表达阳性率分别为(31.14±8.96)%、(54.76±9.53)%,差异有统计学意义(t=7.35,P<0.05)。结论 P-gp、MRP1、LRP和GST-π在喉鳞状细胞癌中表达存在差异性;放射治疗可以增加喉鳞状细胞癌耐药基因蛋白过表达,特别是放疗后P-gp和MRP1的过表达影响喉癌化疗方案的制定。
Objective To investigate the expression of P-glycoprotein, multidrug resistance-related protein (MRP1), lung resistance-related protein (LRP) and glutathione-S-transferase (GST- The expression of tumor tissue before and after radiotherapy and the effect of radiotherapy on the drug resistance gene in. Methods Sixty-six patients with advanced laryngeal carcinoma were collected, including 8 cases of poorly differentiated squamous cell carcinoma and 68 cases of well-differentiated squamous cell carcinoma. Immunohistochemistry was used to detect the expression of P-gp in different types of laryngeal carcinoma and before and after radiotherapy , MRP1, LRP and GST-π expression intensity and distribution, analysis of its expression. Results The laryngeal squamous cell carcinoma had primary drug resistance. The expression of P-gp was strongly and strongly positive in the two pathological types of laryngeal carcinoma with poorly differentiated squamous cell carcinoma and moderately well-differentiated squamous cell carcinoma. Compared with MRP1, LRP and GST-π (F = 8.0839, P = 0.0438). The expression of MRP1 in poorly differentiated squamous cell carcinoma was significantly higher than that in moderately well-differentiated squamous cell carcinoma, the difference was statistically significant (t = 9.575, P <0.001). The positive rates of P-gp, MRP1, LRP and GST-π after radiotherapy were higher than those before radiotherapy. The positive rates of P-gp before and after radiotherapy were 53.26 ± (31.14 ± 8.96)%, (54.76 ± 9.53)%, respectively. The positive rates of MRP1 expression before and after radiotherapy were 7.94%, 79.37 ± 7.73%, respectively The difference was statistically significant (t = 7.35, P <0.05). Conclusions The expression of P-gp, MRP1, LRP and GST-π in laryngeal squamous cell carcinoma is different. Radiation therapy can increase the expression of multidrug resistance gene protein in laryngeal squamous cell carcinoma, especially the expression of P-gp and MRP1 Overexpression affects the development of laryngeal cancer chemotherapy regimen.