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本试验通过建立组织笼感染模型,研究马波沙星在多杀性巴氏杆菌感染猪组织液中的药代动力学。向组织笼中注射0.5 mL对数期多杀性巴氏杆菌(5×10~8CFU/mL),感染后第24小时,对猪按体重单剂量肌内注射1.0 mg/kg和2.5 mg/kg马波沙星,按预定的时间点采集组织液样品,采用高效液相色谱(HPLC)测定组织液中马波沙星的浓度。用Win Nonlin5.2.1软件提供的非房室模型处理组织液药物浓度-时间数据。结果,不同剂量给药的主要药物动力学参数如下:1 mg/kg剂量给药:t_(max)=4 h±1.41 h,C_(max)=0.30 g/mL±0.06g/mL,AUC=9.96 g/mL·h±1.54 g/mL·h,t_(1/2β)=22.92 h±6.83 h;2.5 mg/kg剂量给药:t_(max)=5 h±1.41 h,C_(max)=0.71 g/mL±0.09 g/mL,AUC=21.06 g/mL·h±3.69 g/mL·h,t_(1/2β)=20.91 h±8.57 h。结果表明,马波沙星在多杀性巴氏杆菌感染猪组织液中的峰浓度较高,达峰时间较慢,消除半衰期较长。
This experiment through the establishment of tissue cage infection model to study Marbofloxacin Pasteurella multocida infection in pig tissue pharmacokinetics. The mice were injected intraperitoneally with 0.5 mL logarithmic Pasteurella multocida (5 × 10 ~ 8 CFU / mL) and 24 h after infection, pigs were injected with 1.0 mg / kg and 2.5 mg / kg intramuscularly Marbofloxacin, tissue samples were collected at predetermined time points, and the concentration of marbofloxacin in tissue fluid was determined by high performance liquid chromatography (HPLC). Tissue fluid drug concentration-time data were processed using the non-compartmental model provided by Win Nonlin 5.2.1 software. As a result, the main pharmacokinetic parameters for different doses were as follows: 1 mg / kg Dosing: t max 4 h ± 1.41 h C max 0.30 g / mL ± 0.06 g / mL AUC = 9.96 g / mL · h ± 1.54 g / mL · h, t 1/2 × 22.92 h ± 6.83 h; 2.5 mg / kg dosing: t max = 5 h ± 1.41 h, = 0.71 g / mL ± 0.09 g / mL, AUC = 21.06 g / mL · h ± 3.69 g / mL · h, t 1/2 (1/2) = 20.91 h ± 8.57 h. The results showed that the peak concentration of marbofloxacin in Pasteurella multocida infected swine tissue was higher, the peak time was slower and the elimination half-life was longer.