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目的预测及比较分析猪带绦虫乳酸脱氢酶A(Taenia solium lactate dehydrogenase A,TsLDH-A)和乳酸脱氢酶B(Taenia solium lactate dehydrogenase B,TsLDH-B),用于指导其生物学功能的研究。方法利用生物信息网站如美国国家生物技术信息中心(NCBI,http://www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http://ca.expasy.org/)中有关基因和蛋白的序列和结构信息分析的各种工具,结合其它生物信息学分析软件包,从猪带绦虫成虫全长cDNA质粒文库中识别LDH-A和LDH-B的全长编码基因并对其结构与功能进行生物信息学预测分析。结果两序列都是包含完整开放阅读框的全长基因,推导出的氨基酸序列与其它物种LDH-A或LDH-B同源基因的氨基酸序列的一致性均大于50%。两者编码的蛋白在编码的氨基酸数目(331)、蛋白的理化性质、L-乳酸脱氢酶结构域、构成LDH酶催化中心的关键氨基酸、包含LDH活性位点的线性表位、无亚细胞定位等方面是一致的,但两者在翻译后的修饰位点、3个跨膜区和其他线性表位方面既相似也有区别。结论应用生物信息方法从猪带绦虫成虫cDNA文库中筛选出了TsLDH-A和TsLDH-B的cDNA全长序列,并预测和比较了两者结构与功能方面的信息,为进一步研究所编码蛋白的功能奠定了基础。
Objective To predict and compare Taenia solium lactate dehydrogenase A (TSLDH-A) and Tailed lactate dehydrogenase B (TLDH-B) to guide their biological functions the study. Methods Bioinformatics websites such as the National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.nih.gov/) and the Protein Analysis Expert System of the Swiss Institute of Bioinformatics (ExPASY, http: // ca (www.expasy.org/), and other bioinformatics analysis software packages to identify LDH-A and LDH-B from full-length cDNA plasmid library of adult Taenia solium Full-length coding gene and its structure and function of bioinformatics prediction analysis. Results Both sequences were full-length genes containing the complete open reading frame. The deduced amino acid sequences were more than 50% identical to the amino acid sequences of LDH-A or LDH-B homologues of other species. The number of amino acids encoded by the two proteins (331), the physicochemical properties of the protein, the L-lactate dehydrogenase domain, the key amino acids that make up the LDH enzyme catalytic center, the linear epitopes containing LDH active sites, Localization and so on, but both are similar and different in the post-translational modification sites, three transmembrane regions and other linear epitopes. Conclusion The full-length cDNA sequences of TsLDH-A and TsLDH-B were screened from cDNA library of adult Taenia solium using bioinformatics methods. The structure and function of both genes were predicted and compared. In order to further study the relationship between the encoded proteins Function laid the foundation.