Flow cytometry assay of myeloid dendritic cells (mDCs)in peripheral blood during acute hepatitis C:P

• 作 者: Alessandro Perrella Luigi Atripaldi Pasquale Bellopede Tommaso Patarino Costanza Sbreglia Giovanni Tarantino Paolo Sorrentino Paolo Conca Luca Ruggiero Oreste Perrella
• 机 构: Department Infectious Disease and Immunology Hospital D.Cotugno,Naples,Italy,Department Infectious D
• 出 处: World Journal of Gastroenterology
• 发表日期: 2006年07期
• 关键词: myeloid cytometry contrary infective absolute remaining gating illness involveme

AIM:To asses the expression of myeloid dendritic cells(CD11c+)subset during acute HCV hepatitis and itspossible involvement in natural history of the infection.METHODS:We enrolled 11 patients with acute hepatitisC(AHC)(Group A),10 patients with acute hepatitis A(AHA)(as infective control-Group B)and 10 healthydonors(group C)in this study.All patients underwentselective flow cytometry gating strategies to assess theperipheral number of the myeloid dendritic cells(mDCs)to understand the possible role and differences duringacute hepatitis.RESULTS:Eight of 11 patients with acute HCV hepatitisdid not show any increase of mDCs compared to healthyindividuals,while a significant decrease of mDCs wasfound in absolute cell count(z=-2.37;P<0.05)andpercentage(z=-2.30;P<0.05)as compared with AHA.On the contrary,The remaining three patients of thegroup A had a higher mDCs number and percentage asoccur in group B.Interestingly,after six months,thosepatients did not show any increase of mDCs subset werechronically infected,while the three subjects with anincrease of peripheral mDCs,as in HAV acute infection,resolved the illness.CONCLUSION:The lack of increase of mDCs duringacute hepatitis C might be an important factor involvedin chronicization of the infection.

AIM: To asses the expression of myeloid dendritic cells (CD11c +) subset during acute HCV hepatitis and its likely involvement in natural history of the infection. METHODS: We enrolled 11 patients with acute hepatitis C (AHC) (Group A), 10 patients with acute hepatitis A (AHA) (as infective control-Group B) and 10 healthydonors (group C) in this study. All patients underwentselective flow cytometry gating strategies to assess theperipheral number of the myeloid dendritic cells (mDCs) to understand the possible role and differences duringacute Eight of 11 patients with acute HCV hepatitis did not show any increase of mDCs compared to healthy individuals, while a significant decrease of mDCs was found in absolute cell count (z = -2.37; P <0.05) andpercentage (z = -2.30; P <0.05) as compared with AHA. Of the contrary, The remaining three patients of the group A had a higher mDCs number and percentage asoccur in group B. Interestingly, after six months, thosepatients did not show any increase of mDCs subset we rechronically infected, while the three subjects with anincrease of peripheral mDCs, as in HAV acute infection, resolved the illness. CONCLUSION: The lack of increase of mDCs duringacute hepatitis C might be an important factor involved in chronicization of the infection.