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目的探讨4.10 MPa氢氧暴露对大鼠氧化应激的影响。方法无特定病原体级健康成年雄性SD大鼠30只随机分为对照组、氦氧组和氢氧组。3组大鼠分别在动物加压舱内的常压常氧空气、4.10 MPa氦氧及4.10 MPa氢氧环境中暴露24 h。出舱后处死大鼠,分离脑、肺和肝组织,比色法测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OhdG)水平。结果对照组、氦氧组和氢氧组3组大鼠脑组织SOD、GSH-Px活力及GSH水平分别比较,差异均有统计学意义[(153.09±37.62)vs(114.97±23.38)vs(162.31±36.03)mmol/(min·gprot),(1 042.27±248.32)vs(746.77±188.13)vs(1 040.73±192.41)μmol/(min·gprot),(2.45±0.64)vs(1.46±0.36)vs(2.06±0.49)mg/gprot,P<0.05,P<0.05,P<0.01];氦氧组脑组织SOD、GSH-Px活力及GSH水平均低于对照组(P<0.05),氢氧组脑组织SOD、GSH-Px活力和GSH水平均高于氦氧组(P<0.05)。3组大鼠肺组织GSH-Px活力和GSH水平分别比较,差异均有统计学意义[(437.29±46.62)vs(358.34±31.49)vs(446.40±55.52)μmol/(min·gprot),(2.30±0.55)vs(0.98±0.13)vs(1.67±0.30)mg/gprot,P<0.01,P<0.05];氦氧组肺组织GSH-Px活力和GSH水平均低于对照组(P<0.05),氢氧组肺组织GSH-Px活力高于氦氧组(P<0.05)。3组大鼠肝组织SOD和GSH-Px活力分别比较,差异均有统计学意义[(101.31±9.67)vs(93.99±6.13)vs(112.23±11.41)mmol/(min·gprot),(325.28±34.39)vs(276.67±22.29)vs(341.05±31.74)μmol/(min·gprot),P<0.01];氦氧组肝组织GSH-Px活力低于对照组(P<0.05),氢氧组肝组织中SOD和GSH-Px活力均高于氦氧组(P<0.05)。氢氧组脑、肺和肝3种组织SOD、GSH-Px活力及GSH水平分别与对照组相应组织比较,差异均无统计学意义(P>0.05)。3组组间脑、肺和肝3种组织的MDA及8-OhdG水平分别比较,差异均无统计学意义(P>0.05)。结论 4.10 MPa氢氧暴露大鼠的氧化应激指标未显现出明显改变,与氢对高氧毒性的抑制作用有关。从氧化应激的角度看,高压氢氧环境比高压氦氧环境具有更好的生理安全性。
Objective To investigate the effects of hydrogen and oxygen exposure at 4.10 MPa on oxidative stress in rats. Methods Thirty healthy adult male Sprague - Dawley rats without specific pathogen were randomly divided into control group, heliox group and hydrogen and oxygen groups. The rats in the three groups were exposed to normoxic air under atmospheric pressure, 4.10 MPa helium oxygen and 4.10 MPa hydrogen atmosphere for 24 h respectively. Rats were sacrificed after exiting the capsule, and brain, lung and liver tissues were isolated. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and reduced glutathione GSH), malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OhdG). Results The SOD, GSH-Px activities and GSH levels in brain tissue of three groups of rats in the control group, the helium oxygen group and the oxy-hydrogen group were significantly different (153.09 ± 37.62 vs 114.97 ± 23.38 vs 162.31 ± 36.03) mmol / (min · gprot), (1042.27 ± 248.32) vs (746.77 ± 188.13) vs (1.040.73 ± 192.41) μmol / min · gprot vs 2.46 ± 0.36 vs 1.46 ± 0.36 vs (2.06 ± 0.49) mg / gprot, P <0.05, P <0.05, P <0.01]. The activity of SOD, GSH-Px and the level of GSH in brain tissue of He-oxygen group were lower than those in control group Brain SOD, GSH-Px activity and GSH levels were higher than helix oxygen group (P <0.05). The levels of GSH-Px and GSH in the lung tissue of the three groups were significantly different (437.29 ± 46.62 vs 358.34 ± 31.49 vs 446.40 ± 55.52 μmol / min / gprot, (P <0.05). Compared with the control group, the GSH-Px activity and GSH level in lung tissue of He-oxygen group were significantly lower than those in control group (P <0.05 vs 0.55 vs 0.98 ± 0.13 vs 1.67 ± 0.30 mg / gprot, P < (P <0.05). The activity of GSH-Px in lungs of oxytocin group was higher than that of helium-oxygen group (P <0.05). The activities of SOD and GSH-Px in the liver tissue of the three groups were significantly different ([101.31 ± 9.67 vs 93.99 ± 6.13 vs 112.23 ± 11.41 mmol / (min · gprot, (325.28 ± P <0.01). The activity of GSH-Px in the Hepatic oxygen group was lower than that in the control group (P <0.05) The activities of SOD and GSH-Px in tissues were higher than that in helium-oxygen group (P <0.05). The activities of SOD, GSH-Px and the levels of GSH in brain tissue, lung and liver of oxy-gen group were not significantly different from those in the control group (P> 0.05). The levels of MDA and 8-OhdG in the brain, lung and liver tissues among the three groups were not significantly different (P> 0.05). Conclusions The oxidative stress index of rats exposed to hydrogen peroxide at 4.10 MPa showed no significant change, which is related to the inhibitory effect of hydrogen on hyperoxic toxicity. From the point of view of oxidative stress, the high pressure hydrogen environment has better physiological safety than the high pressure helium oxygen environment.