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为了观察新生大鼠缺氧缺血性脑损伤（HIE）后不同时间血浆血管活性肠肽和P物质兔疫活性物质（ir-VIP、ir-SP）含量的动态变化,将7日龄SD大鼠随机分为正常对照组（C）、假处理组（B）和HIE组（A）,采用一侧颈总动脉结扎联合低氧吸入形成HIE,放射免疫分析法测定各组伤后即刻24、48和72h血浆ir-VIP和ir-SP水平的动态变化。结果表明,血浆ir-VIP水平A1、A2、A3组分别显著低于B1、B2、B3组;ir-SP水平,A2、A3、A4组分别显著高于B2、B3、B4组。B、C组之间ir-VIP和ir-SP含量相差不显著,证实VIP、SP参与HIE的病理生理过程,血浆含量的显著变化提示其在HIE中起作用。
In order to observe the dynamic changes of plasma vasoactive intestinal peptide and iris-active substance (ir-SP) content in neonatal rats after hypoxic-ischemic brain damage (HIE) at different time, The rats were randomly divided into normal control group (C), sham-operation group (B) and HIE group (A). One side of common carotid artery ligation and hypoxia inhalation were used to form HIE. Radioimmunoassay Dynamic changes of plasma ir-VIP and ir-SP levels at 48 and 72 hours. The levels of ir-SP in group A1, A2 and A3 were significantly lower than those in groups B1, B2 and B3, respectively. The level of ir-SP in group A2, A3 and A4 were significantly higher than those in groups B2, B3 and B4 respectively. The difference of ir-VIP and ir-SP between group B and group C was insignificant. It was confirmed that VIP and SP were involved in the pathophysiological process of HIE. The significant change of plasma content suggested that VIP and SP play a role in HIE.