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乳兔肾原代细胞和MERN人胚肾传代细胞,用液氮-196℃作制冷剂,低温冰冻24小时,经各种不同降温率及复温率,加入~3H—胸腺嘧啶核苷,经测定结果,加入10%二甲基亚砜,降温率从+20℃降至-20℃时为1±0.1℃/分钟,-20℃以下为375℃/分钟;复温率从-196℃上升至+20℃时为130℃/分钟,其脉冲数虽较未冷冻为低,但较不加二甲基亚砜为高,有显著性差异(P<0.01)。提示细胞在二甲基亚砜溶液中,经超低温袭击仍有较高的复制脱氧核糖核酸功能。
Rabbit kidney primary cells and MERN human embryonic kidney passage cells, with liquid nitrogen -196 ℃ as a refrigerant, low temperature freezing 24 hours, by a variety of cooling rate and rewarming rate, by adding ~ 3H-thymidine, by The results of the measurement were as follows: adding 10% dimethylsulfoxide, decreasing the temperature drop rate from + 20 ° C to -20 ° C to 1 ± 0.1 ° C / min and decreasing to -20 ° C to 375 ° C / min; the rewarming rate increased from -196 ° C To + 20 ° C at 130 ° C / min. Although the pulse number was lower than that without freezing, it was significantly lower than that without dimethyl sulfoxide (P <0.01). Tip cells in dimethyl sulfoxide solution, the cryogenic attack by cryogenic still have a higher copy of DNA function.