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目的：研究维拉帕米（Ｖｅｒ）是否能抑制去甲肾上腺素（ＮＥ）诱导的培养大鼠心肌细胞β受体下调及其可能机制．方法：β受体密度用［３Ｈ］ＤＮＡ放射配基标记法，细胞内游离钙用钙离子荧光探针Ｆｕｒａ２ＡＭ法测定．结果：Ｖｅｒ能明显降低培养大鼠心肌细胞内游离钙水平，增加β受体密度；ＮＥ增加细胞内游离钙水平，降低β受体密度；Ｖｅｒ能明显抑制ＮＥ引起的细胞内游离钙增加和β受体密度的降低．结论：Ｖｅｒ能增加正常心肌细胞β受体密度，抑制ＮＥ产生的心肌β受体下调．
AIM: To investigate whether verapamil can inhibit the downregulation of β receptors in cultured rat cardiomyocytes induced by norepinephrine (NE) and its possible mechanism. Methods: The β receptor density was measured by [3H] DNA radioligand labeling method and intracellular free calcium was measured by Fura2AM method with calcium ion fluorescence probe. RESULTS: Ver significantly decreased intracellular free calcium levels and β receptor density in cultured rat myocardial cells; NE increased intracellular free calcium levels and decreased β receptor density; Ver significantly inhibited NE-induced intracellular free calcium increase and β Receptor density decreased. Conclusion: Ver increased the density of β receptor in normal cardiomyocytes and inhibited the down-regulation of β receptor in NE induced by NE.