Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA s

• 作 者: Ziwei Chen Xuying Duan Hua Wei Suming Tang Chidong Xu Yanlei Li Yifu Guan Guojie Zhao
• 机 构: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110122, China;A
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2018年5期
• 关键词: galactose assay rolling circle amplification repressor silver staining

Oligonucleotides were screened for strongly silver-stained repetitive sequences. An ’AG’-clustered purine sequence showed strong staining, and the staining density can be compromised by disrupting the continuity of the ’AG’-clustered sequence. The staining-favored sequence was then employed in rolling circle amplification (RCA) for its product detection. A tube-staining method was developed for convenient and visual RCA assay. Moreover, by introducing GaIR into RCA, D-galactose was detected by RCA tube-staining with naked eyes without any equipment. About 10 mM D-galactose can be easily identified, and the detection of D-galactose was specific in comparison with that of several other monosaccharides.