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本文考察了罗非鱼皮胶原酶解物(TSCH)对双醛淀粉(DAS)交联明胶膜理化性质的影响,探讨了膜对人皮肤永生化角质(Ha Cat)细胞的相容性。结果发现,TSCH添加量从0增加至16%时,DAS交联明胶膜的抗拉伸强度从55.00 MPa下降至24.85 MPa,而断裂延伸率随着TSCH添加量的增加呈现先增大后减小的趋势。DAS交联明胶膜在30℃水中的溶解率为33.10%,当膜中TSCH含量超过4%后,膜的溶解率接近100%。然而,不管TSCH添加量是多少,经过240 min后膜中含有的TSCH全部释放到水中。SDS-PAGE的结果表明,在DAS交联明胶膜中添加的TSCH会阻碍明胶蛋白分子的相互交联作用。另一方面,将Ha Cat细胞培养于DAS交联明胶膜时,Ha Cat细胞活力只有11.39%,当DAS交联明胶膜中含有8%的TSCH时,Ha Cat细胞活力可以达到108.20%。以上结果表明,添加TSCH的DAS交联明胶膜在化妆品领域将具有一定的应用潜力。
In this paper, the effects of TSCH on the physico-chemical properties of crosslinked gelatin films of dialdehyde starch (DAS) were investigated, and the compatibility of the film on human dermal immortalized keratinocytes (HaCat) was investigated. The results showed that the tensile strength of DAS crosslinked gelatin film decreased from 55.00 MPa to 24.85 MPa when the amount of TSCH was increased from 0 to 16%, while the elongation at break increased first and then decreased with the increase of TSCH the trend of. The dissolution rate of DAS cross-linked gelatin film in water at 30 ℃ is 33.10%. When the TSCH content in the film exceeds 4%, the dissolution rate of the film is close to 100%. However, no matter how much TSCH was added, the TSCH contained in the membrane was released into the water after 240 min. The results of SDS-PAGE showed that addition of TSCH in DAS cross-linked gelatin film hindered the mutual cross-linking of gelatin protein molecules. On the other hand, Ha Cat cells had a viability of only 11.39% when cultured on DAS cross-linked gelatin membranes, and HaCaT cells reached 108.20% viability when DCH cross-linked gelatin membranes contained 8% TSCH. The above results show that adding TSCH DAS crosslinked gelatin film in the field of cosmetics will have some potential applications.