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本文报道狭叶肉桂(Cassia angustifolia)叶经水粗提、离心、透析得2.5％粗多糖,依次用DEAE-凝胶和葡聚酰-S-400凝胶柱分离,分别用pH6磷酸盐缓冲液和0.1mol/LNaC1洗脱。再依次用0.05mol/L、0.5mol/L和2mol/L三氟乙酸温和水解,水解物用Bio凝胶-P-2柱分离,水洗脱,最后获得名为SI_A的酸性多糖。SI_A经用甲基化分析。以气一质联用仪配合鉴定甲基化物,并测定了结构。 SI_A酸性多糖的抗肿瘤活性研究表明,对CDI小鼠肉瘤-180抑制率可达51％。它的作用机制并不显示细胞毒活性,而是对宿
In this paper, Cassia angustifolia leaves were crudely extracted with water, centrifuged, and dialyzed to obtain 2.5% of crude polysaccharides. They were separated on DEAE-gel and Gluco-S-400 gel columns in turn and pH 6 phosphate buffers were used respectively. Elution with 0.1 mol/L NaC1. This was further hydrolyzed sequentially with 0.05 mol/L, 0.5 mol/L, and 2 mol/L trifluoroacetic acid. The hydrolysate was separated on a Biogel-P-2 column and eluted with water to give an acidic polysaccharide called SI_A. SI_A was analyzed with methylation. The methylation product was identified with a gas-mass spectrometer, and the structure was determined. The anti-tumor activity of SI_A acid polysaccharides has shown that the inhibition rate of C180 mouse sarcoma-180 can reach 51%. Its mechanism of action does not show cytotoxic activity, but rather