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运用高效区带毛细管电泳法以pH2.7浓度配制为15mmol/L柠檬酸-20mmol/L柠檬酸三钠作电泳缓冲液同时测定了生鲜乳蛋白和大豆分离蛋白。结果显示β-CN峰面积和迁移时间的相对标准偏差(RSD)分别为3.01%和0.62%,κ-CN峰面积和迁移时间的RSD分别为2.03%和0.49%,大豆分离蛋白峰面积和迁移时间的RSD分别小于5.12%和0.48%,均满足定性和定量分析的要求。建立了利用蛋白峰面积比例关系间接测定生鲜乳中大豆分离蛋白的分析方法,其中β-CN和κ-CN检测限分别为0.17mg/L和0.23mg/L,回收率为99.37%和99.23%,大豆分离蛋白检测限和回收率分别为5.73mg/L和87.44%.
The high performance liquid chromatography with capillary electrophoresis was used to determine the content of fresh milk protein and soy protein isolate at the concentration of 15 mmol / L citric acid -20 mmol / L trisodium citrate as electrophoresis buffer at pH2.7. The results showed that the relative standard deviations (RSDs) of peak area and migration time of β-CN were 3.01% and 0.62%, respectively. The RSDs of peak area and migration time were 2.03% and 0.49%, respectively. The RSDs of time are less than 5.12% and 0.48% respectively, which meet the requirements of qualitative and quantitative analysis. The analytical method was established for the indirect determination of soy protein isolate from fresh milk using the ratio of protein peak area. The detection limits of β-CN and κ-CN were 0.17mg / L and 0.23mg / L respectively, and the recoveries were 99.37% and 99.23% %, The detection limit of soy protein isolate and recovery were 5.73mg / L and 87.44% respectively.