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目的观察不同浓度胡桃醌(Juglone)对SKOV3细胞侵袭转移能力及基质金属酶MMP-2表达的影响。方法人卵巢癌细胞SKOV3为研究对象,随机分为对照组和Juglone组,Juglone组再按照Juglone剂量不同,分为25,50,100μmol·L-1三组。以细胞划痕试验和Transwell小室法检测SKOV3细胞侵袭力和迁移的变化,Western blot法检测MMP-2蛋白表达的变化。结果细胞侵袭力测定显示,Juglone各组侵袭过Matrigel胶的细胞数显著低于对照组(0.649±0.088,0.488±0.021,0.291±0.035,vs 0.880±0.0032均P<0.05),Juglone组细胞侵袭能力较对照组显著降低;细胞迁移实验结果显示,Juglone各组穿过滤膜的细胞数显著低于对照组(0.607±0.064,0.419±0.018,0.165±0.014,vs 0.896±0.048,均P<0.05);细胞划痕实验显示,Juglone各组创伤愈合率显著低于对照组[(89.21±2.64)%,(48.55±4.04)%,(20.19±1.09)%,vs100%,均P<0.05]。Western blot检测MMP-2在SKOV3细胞的蛋白表达水平,显示Juglone各组较对照组显著降低(P<0.05)。结论胡桃醌可能通过抑制MMP-2表达,抑制卵巢癌细胞侵袭转移能力。
Objective To observe the effects of different concentrations of Juglone on the invasion and metastasis of SKOV3 cells and the expression of matrix metalloproteinase-2 (MMP-2). Methods Human ovarian cancer cell line SKOV3 was randomly divided into control group and Juglone group. Juglone group was divided into three groups (25, 50 and 100μmol·L-1) according to the dose of Juglone. The invasion and migration of SKOV3 cells were detected by cell scratch assay and Transwell chamber assay. The expression of MMP-2 protein was detected by Western blot. Results The invasiveness of Juglone cells in each group was significantly lower than that in the control group (0.649 ± 0.088,0.488 ± 0.021,0.291 ± 0.035, vs 0.880 ± 0.0032, P <0.05). The cell invasion ability of Juglone group The results of cell migration showed that the number of Juglone cells in each group was significantly lower than that in the control group (0.607 ± 0.064, 0.419 ± 0.018, 0.165 ± 0.014, 0.896 ± 0.048, all P <0.05) Cell scratch test showed that the wound healing rate of Juglone group was significantly lower than that of the control group [(89.21 ± 2.64)%, (48.55 ± 4.04)%, (20.19 ± 1.09)%, vs100%, all P <0.05]. The protein expression of MMP-2 in SKOV3 cells was detected by Western blot, which showed that Juglone group was significantly lower than the control group (P <0.05). Conclusion Juglone may inhibit the invasion and metastasis of ovarian cancer cells by inhibiting the expression of MMP-2.