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本文应用2×2析因实验设计将wistar大鼠分为四组:I~+Se~+,I~+Se~-,I~-Se~+及1~-Se~-。测定了喂养30周大鼠肝脏、大脑组织T_45’—脱碘酶活性以及血清T_4、T_3及rT_3水平。结果表明大鼠肝脏T_45’—脱碘酶活性于低硒组(I~+Se~-和I~-Se~-)明显低于补硒组(I~+Se~+和1~-Se~+),而碘因素对此无影啊;脑组织T_45’—脱碘酶活性在四组中无明显差别。血清T_4、rT_3水平于低碘组明显低于补碘组,T_3变化不明显;而低硒可升高血清T_4水平,并有升高rT_3趋势,碘硒交互作用明显,主要表现补碘低硒(I~+Se~-)可明显升高T_4、rT_3水平,而低碘补硒(I~-Se~+)作用则相反。上述结果提示低硒时肝脏T_45’—脱碘酶活性下降可能是血清T_4、rT_3升高的直接原因,在本实验中碘硒两因素对血清T_3无明显作用。
In this paper, 2 × 2 factorial experimental design wistar rats were divided into four groups: I ~ + Se ~ +, I ~ + Se ~ -, I ~ -Se ~ + and 1 ~ -Se ~ -. The activities of T_45’-deiodinase and the levels of serum T_4, T_3 and rT_3 in the liver and brain of rats fed for 30 weeks were measured. The results showed that the activity of T_45’-deiodinase in rat liver was significantly lower than that in selenium-selenium group (I ~ + Se ~ - and I ~ -Se ~ -) +), While the iodine factor has no effect on this; brain tissue T_45’-deiodinase activity in the four groups no significant difference. Serum levels of T_4 and rT_3 were significantly lower in iodine-deficient group than those in iodine-supplemented group, while T_3 did not change significantly. Low selenium could increase serum T_4 level and increase rT_3 tendency. The interaction between iodine and selenium was obvious. (I ~ + Se ~ -) significantly increased the levels of T_4 and rT_3, while the effect of I ~ -Se ~ + was the opposite. The above results suggest that the decrease of hepatic T_45’-deiodinase activity may be the direct reason for the increase of serum T_4 and rT_3 in low selenium. In this experiment, the two factors of iodine and selenium have no obvious effect on serum T_3.